| 產(chǎn)品編號(hào) | bs-1188R |
| 英文名稱 | FRA2 Rabbit pAb |
| 中文名稱 | fos相關(guān)抗原2抗體 |
| 別 名 | FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; FosL 2; FOSL2; FOSL2_HUMAN; FRA 2; fos-related antigen 2. |
| 研究領(lǐng)域 | 腫瘤 信號(hào)轉(zhuǎn)導(dǎo) 生長因子和激素 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,GuineaPig) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FRA2: 231-326/326 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Fos and Jun dimerize to form Activator Protein 1 (AP1), a transcriptional factor that binds to the 12-O-tetradecanoylphorbol 13 acetate (TPA) response element (TRE) of several cellular and viral genes including human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. Fos and Jun contain the 'leucine-zipper' motif that allows for dimerization and an adjacent basic domain required for biological activity. The functionally active form of Fos is in a heterodimer with a member of the Jun family. While Jun family members can form functional homodimers, studies indicate that Fos family members do not self-associate and therefore do not bind DNA on their own. The various dimers differ in their ability to transactivate AP1 dependent genes. Function: Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts (By similarity). Subunit: Heterodimer Subcellular Location: Nuclear Similarity: Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP (basic-leucine zipper) domain. SWISS: P15408 Gene ID: 2355 Database links: Entrez Gene: 2355 Human Entrez Gene: 421416 Chicken Entrez Gene: 14284 Mouse Omim: 601575 Human SwissProt: P18625 Chicken SwissProt: P15408 Human SwissProt: P47930 Mouse Unigene: 220971 Human Unigene: 596972 Human Unigene: 24684 Mouse Unigene: 10962 Rat Unigene: 163577 Rat FOSL2蛋白對(duì)細(xì)胞因子、生長因子、感染或致癌刺激等生理或病理信號(hào)發(fā)生應(yīng)答,調(diào)節(jié)基因的轉(zhuǎn)錄, 參與細(xì)胞的增殖、分化等過程. FOSL2在腫瘤形成及發(fā)展過程中, 通過促進(jìn)細(xì)胞增殖、抑制分化、促進(jìn)腫瘤細(xì)胞的侵襲和轉(zhuǎn)移等過程發(fā)揮作用. |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Small intestine tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-FRA2 (bs-1188R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band siz
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal go
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal go
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-FRA2 antibody (bs-1188R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The
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