| 產(chǎn)品編號 | bs-0318R |
| 英文名稱 | PADI4 Rabbit pAb |
| 中文名稱 | 關(guān)節(jié)炎相關(guān)基因抗體 |
| 別 名 | EC 3.5.3.15; HL 60 PAD; HL-60 PAD; PAD 4; PAD; PADI 4; PADI 5; PADI H protein; PADI5; PDI 4; PDI 5; PDI4; PDI5; Padi4; PADI4_HUMAN; Peptidyl arginine deiminase type IV; Peptidyl arginine deiminase type V; Peptidylarginine deiminase IV; Protein arginine deiminase; Protein-arginine deiminase type IV; Protein-arginine deiminase type-4; Protein arginine deiminase type 4; Protein arginine deiminase type IV. |
| 研究領(lǐng)域 | 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 新陳代謝 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 74 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PADI4: 401-500/663 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PADI4 (peptidyl arginine deiminase, type IV) catalyzes the deimination of arginine residues of proteins. Down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. subcellular location at cytoplasmic granules. Belongs to the protein arginine deiminase family. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. Function: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils. Tissue Specificity: Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. Post-translational modifications: Autocitrullination at Arg-372 and Arg-374 inactivates the enzyme. DISEASE: Rheumatoid arthritis (RA) [MIM:180300]: An inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=The gene represented in this entry may be involved in disease pathogenesis. It could play a role in increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Similarity: Belongs to the protein arginine deiminase family. SWISS: Q9UM07 Gene ID: 23569 Database links: Entrez Gene: 23569 Human Omim: 605347 Human SwissProt: Q9UM07 Human Unigene: 522969 Human PADI4或稱PAD4 (Protein-arginine deiminase type IV) 是關(guān)節(jié)炎相關(guān)基因，分子量為：74kDa。有學(xué)者認(rèn)為：PADI4也稱Ⅳ型肽基精氨酸脫氨酶，該蛋白與關(guān)節(jié)炎的發(fā)生關(guān)系密切。PADI4 基因能夠合成一種酶，將精氨酸變?yōu)榻勖顾?，?dāng)含桔霉素蛋白質(zhì)的數(shù)量超過正常標(biāo)準(zhǔn)后，人體血液中便會出現(xiàn)攻擊含桔霉素的蛋白質(zhì)的抗體，這種機(jī)理是導(dǎo)致關(guān)節(jié)炎的重要因素之一。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PADI4) Polyclonal Antibody, Unconjugated (bs-0318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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