| 產品編號 | bs-1389R |
| 英文名稱 | Rabbit Anti-Cyclin G antibody |
| 中文名稱 | 周期素G抗體 |
| 別 名 | CCNG 1; CCNG; CCNG1; CYCG1; Cyclin G; CCNG1_HUMAN; Cyclin-G; Cyclin G1; CyclinG1; Cyclin-G1. |
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Specific References (1) | bs-1389R has been referenced in 1 publications.
[IF=0.352] Xu, Qian, et al. "CYCLIN G1 INHIBITS THE PROLIFERATION OF MOUSE ENDOMETRIAL STROMAL CELL IN DECIDUALIZATION."Arch Biol Sci. 2017;69(1):71-81. IF(ICC) ; Mouse.
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| 研究領域 | 細胞生物 染色質和核信號 細胞周期蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclin G: 181-295/295 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive. Function: May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity). Subcellular Location: Nucleus. DNA replication foci after DNA damage. Tissue Specificity: High levels in skeletal muscle, ovary, kidney and colon. Similarity: Belongs to the cyclin family. Cyclin G subfamily. SWISS: P51959 Gene ID: 900 Database links: Entrez Gene: 900 Human Omim: 601578 Human SwissProt: P51959 Human Unigene: 79101 Human Cyclin G的生物功能還沒有完全清楚，他是細胞周期蛋白家族中近來發(fā)現(xiàn)的新成員，也是已知的唯一受到腫瘤抑制基因p53調控的細胞周期蛋白。 近年來經(jīng)研究發(fā)現(xiàn)，Cyclin G可能有促進細胞增長的作用，在細胞增殖和神經(jīng)再生中的可能起到一定的作用。 |
| 產品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (bs-1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(bs-1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (bs-1389R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
