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Rabbit Anti-MTA1  antibody (bs-1412R)  
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產(chǎn)品編號 bs-1412R
英文名稱 Rabbit Anti-MTA1  antibody
中文名稱 腫瘤轉移相關蛋白1抗體
別    名 Metastasis-associated 1; Metastasis associated 1; Metastasis associated gene 1; Metastasis associated gene 1 protein; Metastasis associated protein; Metastasis associated protein MTA 1; Metastasis associated protein MTA1; MTA 1; MTA1_HUMAN.  
Specific References  (2)     |     bs-1412R has been referenced in 2 publications.
[IF=3.298] HLA-A2-Restricted Epitopes Identified from MTA1 Could Elicit Antigen-Specific Cytotoxic T Lymphocyte Response Y Wu  WB ;  Human.  
[IF=1.785] Wenying Liu. et al. Prognostic value of MTA1, SOX4 and EZH2 expression in esophageal squamous cell carcinoma. Exp Ther Med. 2021 Jul;22(1):1-11  IHC ;  Human.  
研究領域 腫瘤  細胞生物  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 81kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MTA1: 601-715/715 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This protein was identified in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this protein has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70 kDa component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this protein suggests that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.

Function:
May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.

Subunit:
Component of the nucleosome-remodeling and histone-deacetylase multiprotein complex (NuRD). Interacts with HDAC1 and ITGB3BP/CENPR. Binds to CSNK1G2 in the cytoplasm.

Subcellular Location:
Cytoplasm and Nucleus.

Tissue Specificity:
Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.

Similarity:
Contains 1 BAH domain. Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain.

SWISS:
Q13330

Gene ID:
9112

Database links:

Entrez Gene: 9112 Human

Entrez Gene: 116870 Mouse

Entrez Gene: 64520 Rat

Omim: 603526 Human

SwissProt: Q13330 Human

SwissProt: Q2KHS8 Mouse

SwissProt: Q8K4B0 Mouse

SwissProt: Q62599 Rat

Unigene: 101448 Human

Unigene: 525629 Human

Unigene: 212577 Mouse

Unigene: 5840 Rat



轉錄調(diào)節(jié)因子(Transcriptin Regulators)
MTA1(Metastasis-associated 1)蛋白是一種蛋白復合物,它具有組蛋白脫乙?;傅幕钚?在組蛋白的脫乙酰基,染色質重塑,轉錄控制的過程中具有重要作用。
產(chǎn)品圖片
Sample: Lung(Mouse) Lysate at 30 ug Primary: Anti- MTA1 (bs-1412R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 81 kD Observed band size: 85 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (bs-1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (bs-1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: transplanted tumor of cervical carcinoma in nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(bs-1412R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(black line):SH-SY5Y. Primary Antibody (green line): Rabbit Anti-MTA1 antibody (bs-1412R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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