| 產(chǎn)品編號 | bs-4012R |
| 英文名稱 | ATG12 Rabbit pAb |
| 中文名稱 | 自噬相關蛋白12抗體 |
| 別 名 | APG12-like; APG12L; ATG12; ATG12 autophagy related 12 homolog; Autophagy 12; Autophagy-related protein 12; HAPG12; Ubiquitin-like protein ATG12; ATG12_HUMAN. |
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Specific References (2) | bs-4012R has been referenced in 2 publications.
[IF=4.966] Hui Che. et al. Melatonin exerts neuroprotective effects by inhibiting neuronal pyroptosis and autophagy in STZ‐induced diabetic mice. Faseb J. 2020 Oct;34(10):14042-14054 WB,IF,IHC ; Mouse, Human.
[IF=2.66] Song, Fuyong, et al. "Involvement of autophagy in tri-ortho-cresyl phosphate-induced delayed neuropathy in hens." Neurochemistry International (2013). WB ;
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| 研究領域 | 腫瘤 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15/21 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Apg12: 101-140/140 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998[PubMed 9852036]).[supplied by OMIM]. Function: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Similarity: Belongs to the ATG12 family. SWISS: O94817 Gene ID: 9140 Database links: Entrez Gene: 9140 Human Entrez Gene: 67526 Mouse Omim: 609608 Human SwissProt: O94817 Human SwissProt: Q9CQY1 Mouse Unigene: 264482 Human Unigene: 9852 Mouse Unigene: 6224 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
