| 產(chǎn)品編號 | bs-3940R |
| 英文名稱 | Rabbit Anti-G protein beta 4 antibody |
| 中文名稱 | G蛋白β4抗體/鳥嘌呤核苷酸結(jié)合蛋白β亞基4 |
| 別 名 | G protein beta 4 subunit; GNB 4; GNB4; Guanine nucleotide binding protein (G protein) beta polypeptide 4; Guanine nucleotide binding protein beta 4 subunit; Guanine nucleotide binding protein beta polypeptide 4; Guanine nucleotide binding protein beta subunit 4; Guanine nucleotide binding protein subunit beta 4; Transducin beta chain 4; GBB4_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human G protein beta 4: 51-150/340 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Guanine nucleotide binding proteins (G proteins) are membrane associated, heterotrimeric proteins composed of three subunits: alpha, beta and gamma. The G protein beta subunit assumes a barrel shaped beta propeller structure containing WD40 repeats preceded by an N terminal alpha helix. The beta subunit forms a stable dimer with the gamma subunit. The alpha subunit only contacts the beta subunit in the dimer, lying on the opposite face from the gamma subunit.
G proteins and their receptors (GPCRs) regulate systems as diverse as sensory perception, cell growth and hormonal regulation. Function: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Subunit: G proteins are composed of 3 units, alpha, beta and gamma. Tissue Specificity: Strongly expressed in lung and placenta, whereas it is weakly expressed in brain and heart. Similarity: Belongs to the WD repeat G protein beta family. Contains 7 WD repeats. SWISS: Q9HAV0 Gene ID: 59345 Database links: Entrez Gene: 59345 Human Entrez Gene: 14696 Mouse Omim: 610863 Human SwissProt: Q9HAV0 Human SwissProt: P29387 Mouse SwissProt: Q3THF3 Mouse SwissProt: Q3TY18 Mouse SwissProt: Q3U2F9 Mouse Unigene: 173030 Human Unigene: 139192 Mouse Unigene: 10844 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-G protein beta 4/GNB4 Polyclonal Antibody, Unconjugated(bs-3940R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
