| 產(chǎn)品編號(hào) | bs-4022R |
| 英文名稱 | Rabbit Anti-phospho-PRKAR2A (Ser99) antibody |
| 中文名稱 | 磷酸化蛋白激酶A受體2α亞基抗體 |
| 別 名 | PKA R2 (phospho-Ser96); phospho-PKA R2 (Ser96); phospho-PKA R2 (Ser99); PKA R2; KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha; cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit; |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PKA R2 around the phosphorylation site of Ser99: RV(p-S)VC |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. Mouse Serine 96 (designated Ser 99 in human) is a phosphorylation site on the PKA II?regulatory subunit. Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. Subunit: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Subcellular Location: Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane. Tissue Specificity: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Post-translational modifications: Phosphorylated by the activated catalytic chain. Similarity: Belongs to the cAMP-dependent kinase regulatory chain family. SWISS: P13861 Gene ID: 5576 Database links: Entrez Gene: 5576 Human Entrez Gene: 19087 Mouse Omim: 176910 Human SwissProt: P13861 Human SwissProt: P12367 Mouse Unigene: 631923 Human Unigene: 731573 Human Unigene: 253102 Mouse Unigene: 486392 Mouse Unigene: 9742 Rat |
