| 產(chǎn)品編號(hào) | C-0006 |
| 英文名稱 | Normal Rabbit Serum |
| 中文名稱 | 封閉用正常兔血清（原液） |
| 別 名 | Normal Rabbit serum. Whole serum is obtained from non-immunized (preimmune) healthy Rabbits. The serum is clarified through a 0.45micron low protein binding filter. 兔血清; 兔血清封閉液; 正常血清; 動(dòng)物非免疫血清（兔）; |
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Specific References (3) | C-0006 has been referenced in 3 publications.
[IF=6.6] Lin Liu. et al. Giardia duodenalis and Its Secreted PPIB Trigger Inflammasome Activation and Pyroptosis in Macrophages through TLR4-Induced ROS Signaling and A20-Mediated NLRP3 Deubiquitination. Cells-Basel. 2021 Dec;10(12):3425 IF ; Mouse.
[IF=3.448] Yong Y et al. Electroacupuncture pretreatment attenuates brain injury in a mouse model of cardiac arrest and cardiopulmonary resuscitation via the AKT/eNOS pathway. Life Sci. 2019 Aug 30;235:116821. Other ;
[IF=2.425] Xie W et al. Seasonal expressions of prolactin, prolactin receptor and STAT5 in the scented glands of the male muskrats (Ondatra zibethicus). Eur J Histochem. 2019 Jan 17;63(1). IHC-P ;
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| 保存條件 | Store at -20℃. |
| 產(chǎn)品介紹 |
Rabbit Serum blocking reagent can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications. Application: Rabbit Serum blocking reagent is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding. 封閉血清適用于免疫組化或者免疫熒光實(shí)驗(yàn)中樣本的封閉。可以封閉待檢樣本中與蛋白質(zhì)非特異性結(jié)合的位點(diǎn)，另外還可以封閉樣品中內(nèi)源性的 Fc 片段結(jié)合位點(diǎn)，阻斷抗體與樣品中的 Fc 受體結(jié)合，從而降低背景，減少假陽(yáng)性。在選擇封閉血清時(shí)，要注意封閉用血清中不能含有目標(biāo)蛋白，且與一抗來(lái)源不同，可用與二抗相同來(lái)源的封閉血清。正常兔血清被廣泛應(yīng)用于組織和細(xì)胞染色中非特異性抗體結(jié)合的阻斷，以及抗體的其他應(yīng)用。血清可直接用于阻斷，或作為阻斷緩沖液的成分。 使用方法 免疫組化： 用5-10%正常兔血清封閉（1:10-1:20倍稀釋），室溫孵育5-10分鐘。清除血清，勿洗，滴加適當(dāng)比例稀釋的一抗或一抗工作液，37℃或室溫孵育4℃過(guò)夜。 細(xì)胞涂片： 用5-10%正常兔血清封閉（1:10-1:20倍稀釋），使用 PBS 稀釋（ 0.01mol/L pH7.4），少量點(diǎn)滴：漠過(guò)細(xì)胞涂片即可. |
