| 產品編號 | bs-4095R |
| 英文名稱 | Rabbit Anti-CTNNBIP1 antibody |
| 中文名稱 | β鏈接素相互作用蛋白1抗體 |
| 別 名 | Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Catenin beta interacting protein 1; CTNNBIP 1; ICAT; Inhibitor of beta catenin and Tcf 4; MGC15093; CNBP1_HUMAN. |
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Specific References (1) | bs-4095R has been referenced in 1 publications.
[IF=6.52] Qi, Wei, et al. "Targeting the Wnt‐regulatory protein CTNNBIP1 by microRNA‐214 enhances the stemness and self‐renewal of cancer stem‐like cells in lung adenocarcinomas." STEM CELLS (2015). IHC-F ; Human.
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| 研究領域 | 細胞生物 免疫學 結合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 9kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CTNNBIP1: 3-70/81 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CTNNBIP1 (Catenin, beta interacting protein 1) binds to beta-catenin and prevents the interaction between beta-catenin and TCF transcription factor family members. It is a negative regulator of the Wnt signaling pathway. Function: Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway. Subunit: Binds CTNNB1. Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Similarity: Belongs to the CTNNBIP1 family. SWISS: Q9NSA3 Gene ID: 56998 Database links: Entrez Gene: 56998 Human Entrez Gene: 67087 Mouse Omim: 607758 Human SwissProt: Q9NSA3 Human SwissProt: Q9JJN6 Mouse Unigene: 463759 Human Unigene: 299735 Mouse Unigene: 162132 Rat |
| 產品圖片 |
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- CTNNBIP1 (bs-4095R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 9 kD
Observed band size: 9 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (bs-4095R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CTNNBIP1) Polyclonal Antibody, Unconjugated (bs-4095R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
