| 產(chǎn)品編號(hào) | bs-5323R |
| 英文名稱 | Rabbit Anti-phospho-CD95/FAS (Tyr291) antibody |
| 中文名稱 | 磷酸化載脂蛋白A1抗體 |
| 別 名 | CD95 (phospho Y291); CD95 (phospho Tyr291); p-CD95 (Tyr291); FAS (phospho Y291); FAS (phospho Tyr291); p-FAS (Tyr291); ALPS 1A; ALPS1A; APO 1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD 95; CD 95 antigen; CD95; CD95 antigen; Delta Fas; Delta Fas/APO 1/CD95; Delta Fas/APO1/CD95; FAS 1; FAS 827dupA; Fas AMA; FAS; FAS Antigen; FAS1; FASLG receptor; FASTM; TNF receptor superfamily, member 6; TNFRSF 6; TNFRSF6; TNR6_HUMAN; Tumor necrosis factor receptor superfamily member 6. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human FAS around the phosphorylation site of Tyr291: EA(p-Y)DT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Subunit: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2, 3, 4, 5, 6: Secreted. Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. Post-translational modifications: N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. DISEASE: Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. Similarity: Contains 1 death domain. Contains 3 TNFR-Cys repeats. SWISS: P25445 Gene ID: 355 Database links: Entrez Gene: 355 Human Entrez Gene: 14102 Mouse Omim: 134637 Human SwissProt: P25445 Human SwissProt: P25446 Mouse Unigene: 244139 Human Unigene: 1626 Mouse Unigene: 162521 Rat FAS的凋亡信號(hào)主要是通過與其胞漿區(qū)相關(guān)的死亡結(jié)構(gòu)域蛋白FADD介導(dǎo)的。FAS與FasL結(jié)合后，F(xiàn)ADD一方面通過C端的DD結(jié)合FAS, 另一方面通過N端的DED與Caspase-8 N端DED結(jié)合，通過Caspase-8誘導(dǎo)效應(yīng)性Caspase 蛋白酶的激活，并最終導(dǎo)致細(xì)胞凋亡的發(fā)生。FAS主要表達(dá)于活化淋巴細(xì)胞、單核細(xì)胞、中性粒細(xì)胞和成纖維細(xì)胞等。 Fas又稱作APO-1/CD95，屬TNF受體家族。Fas基因編碼產(chǎn)物為分子量45KD的跨膜蛋白，分布于胸腺細(xì)胞，激活的T和B淋巴細(xì)胞，巨噬細(xì)胞，肝、脾、肺、心、腦、腸、睪丸和卵巢細(xì)胞等。 Fas蛋白與Fas配體結(jié)合后，會(huì)激活caspase，導(dǎo)致靶細(xì)胞走向凋亡。 |
