| 產(chǎn)品編號(hào) | bs-5499R |
| 英文名稱 | Rabbit Anti-phospho-MyoD1 (Ser200) antibody |
| 中文名稱 | 磷酸化肌原調(diào)節(jié)蛋白抗體 |
| 別 名 | MyoD1 (phospho Ser200); MyoD1 (phospho S200); p-MyoD1 (phospho S200); MYOD1(phospho S200);Myogenic differentiation 1; AI503393; bHLHc1; MD1; MGC156574; MYF3; MYOD; MYOD1; PUM; MYOD1_HUMAN. |
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Specific References (1) | bs-5499R has been referenced in 1 publications.
[IF=4.938] Du J et al.
The regulation of skeletal muscle fiber-type composition by betaine is associated with NFATc1/MyoD.J Mol Med (Berl). 2018 Jun 6. WB ; Mouse.
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| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MyoD1 around the phosphorylation site of Ser200: DA(p-S)SP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis. Function: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1 and CDK9. Interacts with DDX5 (By similarity). Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). Ubiquitinated on the N-terminus; which is required for proteasomal degradation. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P15172 Gene ID: 4654 Database links: Entrez Gene: 374048 Chicken Entrez Gene: 4654 Human Entrez Gene: 17927 Mouse Entrez Gene: 443405 Sheep Omim: 159970 Human SwissProt: P16075 Chicken SwissProt: P15172 Human SwissProt: P10085 Mouse SwissProt: P29331 Sheep Unigene: 181768 Human Unigene: 1526 Mouse Unigene: 9493 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Tongue tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Primary: Anti- phospho-MyoD1 (Ser200) (bs-5499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 47 kDa
Blank control: K562.
Primary Antibody (green line): Rabbit Anti-phospho-MyoD1 (Ser200) antibody (bs-5499R)
Dilution:1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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