| 產(chǎn)品編號 | bs-6299R |
| 英文名稱 | Rabbit Anti-DRES17 antibody |
| 中文名稱 | 乳腺癌相關(guān)蛋白DRES17抗體 |
| 別 名 | DRES17; Drosophila-related expressed sequence 17; hprune; HTCD37; Prune homolog; PRUNE like protein; TcD37 homolog; PRUNE_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DRES17: 201-300/453 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PRUNE, the human homologue of the Drosophila gene, is located in 1q21.3, a region highly amplified in human sarcomas, malignant tumours of mesenchymal origin. Human prune (h-prune), a phosphoesterase DHH family appertaining protein, physically interacts with nm23-H1, a metastasis suppressor gene. h-prune is involved in cellular motility and metastasis formation. Metastatic breast cancers were found to overexpress h-prune; h-prune was also found to be highly expressed in colorectal and pancreatic cancers. Hence h-prune is considered useful as a marker for tumor aggressiveness. Function: Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1. Subunit: Homooligomer. Able to homodimerize via its C-terminal domain. Interacts with NME1. Interacts with GSK3; at focal adhesion complexes where paxillin and vinculin are colocalized. Subcellular Location: Cytoplasm. Nucleus. Cell junction, focal adhesion. Note=In some transfected cells a nuclear staining is also observed. Tissue Specificity: Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas. Similarity: Belongs to the PPase class C family. Prune subfamily. SWISS: Q86TP1 Gene ID: 58497 Database links: Entrez Gene: 58497 Human Entrez Gene: 229589 Mouse SwissProt: Q86TP1 Human SwissProt: Q8BIW1 Mouse Unigene: 728223 Human Unigene: 14155 Mouse Unigene: 279287 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DRES17) Polyclonal Antibody, Unconjugated (bs-6299R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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