| 產(chǎn)品編號(hào) | bs-4720R |
| 英文名稱(chēng) | GDF9 Rabbit pAb |
| 中文名稱(chēng) | 生長(zhǎng)分化因子9抗體 |
| 別 名 | GDF9_HUMAN; GDF9; GDF-9; GDF 9; Growth differentiation factor 9. |
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Specific References (2) | bs-4720R has been referenced in 2 publications.
[IF=1.281] Pinar Kacamak. et al. The Effect of Isotretinoin on Oocyte Maturation in Adolescent Female Rats. Gynecol Obstet Inves. 2020;85(4):327-335 IHC ; Rat.
[IF=1.224] Huihui Wang. et al. Regulation of GDF9 and CDKN1B expression in Tibetan sheep testes during different stages of maturity. Gene Expr Patterns. 2021 Nov;:119218 WB,IF ; Sheep.
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| 研究領(lǐng)域 | 心血管 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 生長(zhǎng)因子和激素 細(xì)胞分化 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GDF9: 301-400/454 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GDF 9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF 9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis. Function: Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells. Subunit: Homodimer or heterodimer (Potential). But, in contrast to other members of this family, cannot be disulfide-linked (By similarity). Subcellular Location: Secreted (By similarity). Tissue Specificity: Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level). Post-translational modifications: Phosphorylated; phosphorylation is critical for GDF9 function. In vitro, can be phosphorylated by CK at Ser-325. DISEASE: Note=Altered GDF9 function may be involved in ovarian disorders. Rare variants in GDF9 have been found in patients with premature ovarian failure and mothers of dizygotic twins. Similarity: Belongs to the TGF-beta family. SWISS: O60383 Gene ID: 2661 Database links: Entrez Gene: 2661 Human Entrez Gene: 14566 Mouse Omim: 601918 Human SwissProt: O60383 Human SwissProt: Q07105 Mouse Unigene: 25022 Human Unigene: 490461 Mouse Unigene: 9714 Mouse Unigene: 42874 Rat GDF9屬于轉(zhuǎn)移生長(zhǎng)因子–β(TGF-β)及骨形態(tài)發(fā)生蛋白（BMP）家族成員。 |
| 產(chǎn)品圖片 |
Sample: Ovary (Mouse) Lysate at 40 ug
Primary: Anti-GDF9 (bs-4720R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat ovarian); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A
Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-GDF9 antibody (bs-4720)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 0.2μg /test.
Protocol
Blank control (Black line): Molt4 (Black).
Primary Antibody (green line):Rabbit Anti-GDF9 antibody (bs-4720R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-
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