| 產(chǎn)品編號 | bs-9878R |
| 英文名稱 | Rabbit Anti-Cyclophilin 40/PPID antibody |
| 中文名稱 | 親環(huán)蛋白（親環(huán)素）40抗體 |
| 別 名 | 40 kDa peptidyl prolyl cis trans isomerase; 40 kDa peptidyl prolyl cis trans isomerase D; 40 kDa peptidyl-prolyl cis-trans isomerase; Cyclophilin D; Cyclophilin D; Cyclophilin related protein; Cyclophilin-40; Cyclophilin-related protein; Cyclophilin40; CyclophilinD; CYP 40; CYP 40; CYP-40; CYP40; CYP40; CYPD; CYPD; MGC33096; Peptidyl Prolyl Isomerase D; Peptidyl Prolyl Isomerase D; Peptidyl-prolyl cis-trans isomerase D; Peptidylprolyl isomerase D; PPIase; PPIase D; PPID; PPID; PPID_HUMAN; Rotamase; Rotamase D. |
| 研究領(lǐng)域 | 細胞生物 轉(zhuǎn)錄調(diào)節(jié)因子 轉(zhuǎn)運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclophilin D: 281-370/370 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cyclophilins are conserved, ubiquitous and abundant cytosolic peptidyl-prolyl cis-trans isomerases that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins. Human cyclophilin A (CyPA), an intracellular protein of 165 amino acids, is the target of cyclosporin A (CsA) and is encoded by a single unique gene conserved from yeast to humans. Cyclophilin B (CyPB) is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. Cyclophilin D (CyP40) is a widely expressed cytoplasmic protein that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It is a widely expressed cytoplasmic protein. Function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Subunit: Binds ESR1. Subcellular Location: Cytoplasm. Tissue Specificity: Widely expressed. Similarity: Belongs to the cyclophilin-type PPIase family. PPIase D subfamily. Contains 1 PPIase cyclophilin-type domain. Contains 3 TPR repeats. SWISS: Q08752 Gene ID: 5481 Database links: Entrez Gene: 5481 Human Entrez Gene: 67738 Mouse Omim: 601753 Human SwissProt: Q08752 Human SwissProt: Q9CR16 Mouse Unigene: 183958 Human Unigene: 295252 Mouse Unigene: 136915 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse NIH/3T3 cell lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human 293T cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human K562 cell lysates
Lane 8: Human Jurkat cell lysates
Primary: Anti-Cyclophilin 40/PPID (bs-9878R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20, 40 kDa
Observed band size: 41 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
