| 產(chǎn)品編號 | bs-11019R |
| 英文名稱 | Rabbit Anti-B7-H3/CD276 antibody |
| 中文名稱 | CD276抗體 |
| 別 名 | B7H3; 4Ig B7 H3; 4Ig-B7-H3; AU016588; B7 H3; B7 homolog 3; B7H3; B7RP-2; CD_antigen=CD276; CD276 antigen; CD276; Costimulatory molecule; PSEC0249; UNQ309/PRO352; CD276_HUMAN. |
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Specific References (1) | bs-11019R has been referenced in 1 publications.
[IF=4.96] Nan Cheng. et al. B7-H3 augments the pro-angiogenic function of tumor-associated macrophages and acts as a novel adjuvant target for triple-negative breast cancer therapy. Biochem Pharmacol. 2021 Jan;183:114298 IF ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 干細胞 腫瘤細胞生物標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD276/B7H3: 101-200/534 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] Function: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Subunit: Interacts with TREML2 and this interaction enhances T-cell activation. Subcellular Location: Membrane; Single-pass type I membrane protein (Probable). Tissue Specificity: Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. Similarity: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains. SWISS: Q5ZPR3 Gene ID: 80381 Database links: Entrez Gene: 80381 Human Entrez Gene: 102657 Mouse Omim: 605715 Human SwissProt: Q5ZPR3 Human SwissProt: Q8VE98 Mouse Unigene: 744915 Human Unigene: 77873 Human Unigene: 5356 Mouse 研究人員檢測CD276在癌癥患者來源的內(nèi)皮細胞中的表達情況，在結(jié)腸癌、肺癌、乳腺癌、食道癌和膀胱癌的腫瘤相關(guān)血管細胞中發(fā)現(xiàn)CD276過量表達，而且這些蛋白在腫瘤細胞本身也過量表達。 |
| 產(chǎn)品圖片 |
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
U-2OS(Human) Cell Lysate at 30 ug
Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
JAR(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD276 Polyclonal Antibody, Unconjugated(bs-11019R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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