| 產(chǎn)品編號 | bs-12070R |
| 英文名稱 | Rabbit Anti-P2Y10 antibody |
| 中文名稱 | G蛋白偶聯(lián)嘌呤受體p2y10抗體 |
| 別 名 | G protein coupled purinergic receptor P2Y10; P2ry10; P2Y like receptor; P2Y purinoceptor 10; P2Y-like receptor; P2Y10; P2Y10_HUMAN; Purinergic receptor P2Y G protein coupled 10; Putative P2Y purinoceptor 10. |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human P2Y10: 151-250/339 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nucleotides are important extracellular signaling molecules that mediate several events, such as cell proliferation, differentiation, chemotaxis and cytokine release. The P2 receptor family is activated by the binding of nucleotides and is divided into two subfamilies, designated P2X and P2Y. The P2Y receptor family are G protein-coupled receptors that mediate the effects of extracellular nucleotides, primarily through the activation of phospholipase C (PLC). To some extent, the P2Y receptors can also activate potassium channels or, alternatively, inhibit adenylate cyclase and N-type calcium channels in response to extracellular nucleotides. P2Y10 (purinergic receptor P2Y, G-protein coupled, 10), also known as P2RY10, is a 339 amino acid multi-pass membrane protein that is thought to act as a receptor for purines coupled to G-proteins. P2Y10 is found at low levels in blood leukocytes and is upregulated during promyelocytic cell differentiation. Function: Putative receptor for purines coupled to G-proteins. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Weakly expressed in blood leukocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: O00398 Gene ID: 27334 Database links: Entrez Gene: 27334 Human Entrez Gene: 78826 Mouse Omim: 600515 Human SwissProt: O00398 Human SwissProt: Q8BFU7 Mouse Unigene: 296433 Human Unigene: 74639 Mouse |
| 產(chǎn)品圖片 |
Sample:
HepG2 cell(human) Lysate at 30 ug
Primary: Anti-P2Y10(bs-12070R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 49 kD
Blank control (blue line): raji(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃)
Primary Antibody (green line): Rabbit Anti-P2Y10 antibody (bs-12070R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
