| 產(chǎn)品編號 | bs-14083R |
| 英文名稱 | Rabbit Anti-CSDE1 antibody |
| 中文名稱 | RNA冷休克域結合蛋白E1抗體 |
| 別 名 | Cold shock domain containing E1 RNA binding; Cold shock domain containing protein E1; Cold shock domain-containing protein E1; Csde1; CSDE1_HUMAN; D1S155E; DKFZp779B0247; DKFZp779J1455; FLJ26882; N-ras upstream gene protein; NRAS related; Nras upstream gene protein; NRU; Protein UNR; RP5 1000E10.3; UNR; UNR protein; Upstream of NRAS. |
| 研究領域 | 細胞生物 轉錄調(diào)節(jié)因子 結合蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 89kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CSDE1: 501-600/798 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
UNR is a 798 amino acid protein that localizes to the cytoplasm and contains nine CDS (cold shock) domains. Existing as a component of the multi-protein autoregulatory ribonucleoprotein complex (ARC), UNR functions as an RNA-binding protein that is required for the initiation of rhinovirus RNA translation and is thought to be involved in translationally coupled mRNA turnover. UNR is expressed as two isoforms, designated long and short, and shares over 98% amino acid identity with its rat counterpart, suggesting a conserved role between species. The gene encoding UNR maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Function: RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Subcellular Location: Cytoplasm. Similarity: Contains 9 CSD (cold-shock) domains. SWISS: O75534 Gene ID: 7812 Database links: Entrez Gene: 7812 Human Entrez Gene: 229663 Mouse Omim: 191510 Human SwissProt: O75534 Human SwissProt: Q91W50 Mouse Unigene: 69855 Human Unigene: 277713 Mouse Unigene: 3562 Rat |
| 產(chǎn)品圖片 |
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CSDE1 Polyclonal Antibody, Unconjugated(bs-14083R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CSDE1 Polyclonal Antibody, Unconjugated(bs-14083R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
