| 產(chǎn)品編號 | bs-14497R |
| 英文名稱 | Rabbit Anti-GPR183 antibody |
| 中文名稱 | G蛋白偶聯(lián)受體183抗體 |
| 別 名 | ebi 2; EBI2; EBV induced G protein coupled receptor 2; EBV-induced G-protein coupled receptor 2; ebv-induced gene 2; Epstein-Barr virus-induced gene 2; Epstein Barr virus induced gene 2; epstein-barr virus induced gene 2; Epstein-Barr virus-induced G-protein coupled receptor 2; G protein-coupled receptor 183; G-protein coupled receptor 183; GP183_HUMAN; gpr183; Lymphocyte specific G protein coupled receptor. |
| 研究領域 | 細胞生物 信號轉導 細菌及病毒 G蛋白偶聯(lián)受體 G蛋白信號 細胞膜蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human EBV Induced Gene 2: 201-300/361 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Epstein-Barr virus-induced gene 2 is a 357 amino acid multi pass membrane protein. It is expressed in B-lymphocytes and lymphoid tissues and may function in the modulation of the immune system. Out of the nine genes that are induced by the Epstein-Barr virus, Ebi2 exhibits the highest levels of up-regulation. Ebi2 is a G-protein coupled receptor that signals through the G-protein G錳. Ebi2 contains seven hydrophobic transmembrane regions and a putative N-linked glycosylation site at its extracellular N-terminus. Ebi2 is believed to be involved in regulating the effects of the Epstein-Barr virus on B-lymphocytes. In addition, Ebi2 may play a role mediating normal lymphocyte functions. Function: Orphan receptor that plays a central role during humoral immunity. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha. Subcellular Location: Cell membrane. Tissue Specificity: Expressed abundantly in lymphoid tissues such as spleen and lymph node, and in B- and T-lymphocytes. Also highly expressed in lung, heart and gastrointestinal tract, and weakly expressed in the urogenital system and brain. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P32249 Gene ID: 1880 Database links: Entrez Gene: 1880 Human Entrez Gene: 769212 Chicken Entrez Gene: 100050745 Horse Entrez Gene: 100388743 Marmoset (common) Entrez Gene: 321019 Mouse Omim: 605741 Human SwissProt: P32249 Human SwissProt: Q3U6B2 Mouse Unigene: 784 Human Unigene: 265618 Mouse Unigene: 179130 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Lymph node tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Lymph node tissue lysates
Lane 5: Rat Spleen tissue lysates
Lane 6: Rat Lung tissue lysates
Lane 7: Human HeLa cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human Raji cell lysates
Lane 10: Human K562 cell lysates
Lane 11: Human A549 cell lysates
Primary: Anti-GPR183 (bs-14497R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 38 kDa
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
