| 產品編號 | bs-16640R |
| 英文名稱 | INDOL1 Rabbit pAb |
| 中文名稱 | 吲哚1抗體 |
| 別 名 | 3-dioxygenase 2; 3-dioxygenase-like protein 1; EC 1.13.11.; I23O2_HUMAN; IDO 2; IDO-2; Ido2; INDOL 1; Indoleamine 2; Indoleamine 2, 3 dioxygenase 2; Indoleamine 2, 3 dioxygenase like 1 protein; Indoleamine 2, 3 dioxygenase like protein 1; Indoleamine pyrrole 2, 3 dioxygenase like 1; Indoleamine pyrrole 2, 3 dioxygenase like protein 1; Indoleamine-pyrrole 2. |
| 研究領域 | 細胞生物 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human INDOL1: 251-350/407 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011] Function: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. Similarity: Belongs to the indoleamine 2,3-dioxygenase family. SWISS: Q6ZQW0 Gene ID: 169355 Database links: Entrez Gene: 169355 Human Omim: 612129 Human SwissProt: Q6ZQW0 Human Unigene: 676257 Human |
| 產品圖片 |
Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Mouse Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Rat Spleen tissue lysates
Primary: Anti-INDOL1 (bs-16640R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 60 kD
Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-INDOL1 Polyclonal Antibody, Unconjugated(bs-16640R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
