| 產品編號 | bs-17468R |
| 英文名稱 | SHMT1 Rabbit pAb |
| 中文名稱 | 絲氨酸甲基化酶cSHMT抗體 |
| 別 名 | CSHMT; Cytoplasmic serine hydroxymethyltransferase; cytosolic; GLYC_HUMAN; Glycine hydroxymethyltransferase; Serine hydroxymethyltransferase 1(soluble); Serine hydroxymethyltransferase; Serine hydroxymethyltransferase, cytosolic; Serine methylase; SHMT; Shmt1. |
| 研究領域 | 細胞生物 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SHMT1: 301-400/483 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Function: Interconversion of serine and glycine. Subcellular Location: Cytoplasm. Similarity: Belongs to the SHMT family. SWISS: P34896 Gene ID: 6470 Database links: Entrez Gene: 6470 Human Entrez Gene: 20425 Mouse Omim: 182144 Human SwissProt: P34896 Human SwissProt: P50431 Mouse Unigene: 513987 Human Unigene: 636044 Human Unigene: 364956 Mouse |
| 產品圖片 |
Sample:
U87MG(Human)Cell Lysate at 40 ug
Primary: Anti-SHMT1 (bs-17468R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
