| 產(chǎn)品編號(hào) | bs-20379R |
| 英文名稱 | Rabbit Anti-TLR4 antibody |
| 中文名稱 | Toll樣受體4（CD284）抗體 |
| 別 名 | TLR-4; TLR 4; ARMD10; CD 284; CD284; CD284 antigen; Homolog of Drosophila toll; hTol; Toll (Drosophila) homolog; TOLL; Toll Endotoxin Hyporesponsiveness; Toll like receptor 4; Toll like receptor 4 precursor; TLR4_HUMAN. |
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Specific References (9) | bs-20379R has been referenced in 9 publications.
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| 研究領(lǐng)域 | 心血管 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat,Cow (predicted: Rabbit,Pig,Sheep,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:1000-2000,IHC-P=1:100-500,IHC-F=1:500-1000,IF=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TLR4: 431-530/843 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Post-translational modifications: N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. DISEASE: Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Similarity: Belongs to the Toll-like receptor family. Contains 18 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 TIR domain. SWISS: O00206 Gene ID: 7099 Database links: Entrez Gene: 7099 Human Entrez Gene: 21898 Mouse SwissProt: O00206 Human SwissProt: Q9QUK6 Mouse Unigene: 174312 Human Unigene: 38049 Mouse Toll樣受體4（TLR4）通過激活天然免疫,參與特異性免疫應(yīng)答的啟動(dòng), Toll樣受體4(TLR4)作為一種重要跨膜信號(hào)轉(zhuǎn)導(dǎo)受體參與了內(nèi)毒素誘發(fā)炎癥反應(yīng)的病理過程,對(duì)其調(diào)控機(jī)制的研究日益受到關(guān)注. |
| 產(chǎn)品圖片 |
Sample:
HL-60(Human) Cell Lysate at 40 ug
Primary: Anti-TLR4 (bs-20379R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 105 kD
Tissue/cell:U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TLR4) polyclonal Antibody, Unconjugated (bs-20379R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (TLR4) polyclonal Antibody, Unconjugated (bs-20379R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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