產品編號	bsm-33411M
英文名稱	Mouse Anti-Bcl-2 antibody
中文名稱	Bcl-2單克隆抗體
別名	Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
	Specific References (12) \| bsm-33411M has been referenced in 12 publications. [IF=6.384] Xiaowei Qin. et al. Neddylation inactivation affects cell cycle and apoptosis in sheep follicular granulosa cells. J CELL PHYSIOL. 2022 May 16 WB ; Sheep. PubMed:35578798 [IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078 WB ; Mouse. PubMed:35489469 [IF=5.89] Rentao Zhang. et al. Anti-Gastric Cancer Activity of the Cell-free Culture Supernatant of Serofluid Dish and Lactiplantibacillus plantarum YT013. FRONT BIOENG BIOTECH. 2022; 10: 898240 WB ; Human. PubMed:35677304 [IF=5.572] Miao Song. et al. Mitophagy alleviates AIF-mediated spleen apoptosis induced by AlCl3 through Parkin stabilization in mice. FOOD CHEM TOXICOL. 2023 Jun;176:113762 WB ; Mouse. PubMed:37028746 [IF=4.546] Kuankuan Xiong. et al. Low-Concentration T-2 Toxin Attenuates Pseudorabies Virus Replication in Porcine Kidney 15 Cells. Toxins. 2022 Feb;14(2):121 WB ; Pig. PubMed:35202147 [IF=4.119] Siyu Li. et al. Deltamethrin induces apoptosis in cerebrum neurons of quail via promoting endoplasmic reticulum stress and mitochondrial dysfunction. ENVIRON TOXICOL. 2022 Apr 21 WB ; Bird. PubMed:35446475 [IF=4.102] Yan Li. et al. Inhibition of Nicotinamide adenine dinucleotide phosphate oxidase 4 attenuates cell apoptosis and oxidative stress in a rat model of polycystic ovary syndrome through the activation of Nrf-2/HO-1 signaling pathway. MOL CELL ENDOCRINOL. Mol Cell Endocrinol. 2022 Apr;:111645 WB ; Rat. PubMed:35413388 [IF=4.101] Haixiang Ding. et al. Ailanthone suppresses the activity of human colorectal cancer cells through the STAT3 signaling pathway. Int J Mol Med. 2022 Feb;49(2):1-11 WB ; Human. PubMed:34958109 [IF=2.534] EnShuang Xu. et al. Establishment and transcriptome characterization of tamoxifen-resistant canine mammary gland tumor cells. Res Vet Sci. 2022 Feb;: WB ; Dog. PubMed:35193047 [IF=2.495] Yuge Liao. et al. Inorganic mercury induces liver oxidative stress injury in quails by inhibiting Akt/Nrf2 signal pathway. INORG CHEM COMMUN. 2022 Aug;142:109603 WB ; Bird. PubMed:10.1016/j.inoche.2022.109603 [IF=2.136] Bayarmaa Khadankhuu. et al. 10-O-(N N-Dimethylaminoethyl)-Ginkgolide B Methane-Sulfonate (XQ-1H) Ameliorates Cerebral Ischemia Via Suppressing Neuronal Apoptosis. J Stroke Cerebrovasc. 2021 Sep;30:105987 WB ; Rat. PubMed:34273708 [IF=1.898] Yong-Chang Duan. et al. Swimming Exercise Ameliorates Hypertension-Induced Kidney Dysfunction via Alleviating Renal Interstitial Fibrosis and Apoptosis. Kidney Blood Press R. 2021;46(2):219-228 WB ; Rat. PubMed:33849006
研究領域	腫瘤心血管細胞生物信號轉導細胞凋亡新陳代謝線粒體
抗體來源	Mouse
克隆類型	Monoclonal
克隆號	3F12
交叉反應	Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,GuineaPig,Horse)
產品應用	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	26kDa
細胞定位	細胞核細胞漿細胞膜線粒體
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Bcl-2: 51-150/239
亞型	IgG1
純化方法	affinity purified by Protein G
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產品介紹	The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8). Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Subunit: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex. Subcellular Location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Tissue Specificity: Expressed in a variety of tissues. Post-translational modifications: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PARK2, leading to increase its stability. DISEASE: Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. Similarity: Belongs to the Bcl-2 family. SWISS: P49950 Gene ID: 596 Database links: Entrez Gene: 281020 Cow Entrez Gene: 596 Human Entrez Gene: 12043 Mouse Entrez Gene: 24224 Rat Omim: 151430 Human SwissProt: O02718 Cow SwissProt: P10415 Human SwissProt: P10417 Mouse SwissProt: P49950 Rat Unigene: 150749 Human Unigene: 257460 Mouse Unigene: 9996 Rat
產品圖片	Sample: LOVO Cell (Human) Lysate at 40 ug MCF-7 Cell (Human) Lysate at 40 ug Primary: Anti-Bcl-2 (bsm-33411M) at 1/2 000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 28 kD Sample: LOVO(Human) Cell Lysate at 30 ug Primary: Anti- Bcl-2 (bsm-33411M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (bcl-2) Polyclonal Antibody, Unconjugated (bsm-33411M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, Unconjugated (bs-33411M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

	AbBy Fluor 350 標記
	AbBy Fluor 488 標記
	AbBy Fluor 555 標記
	AbBy Fluor 594 標記
	AbBy Fluor 647 標記
	AP 標記
	APC 標記
	Biotin 標記
	Cy3 標記
	Cy5 標記
	Cy5.5 標記
	Cy7 標記
	FITC 標記
	Gold 標記
	HRP 標記
	PE 標記
	PE-Cy3 標記
	PE-CY5 標記
	PE-CY5.5 標記
	PE-CY7 標記
	RBITC 標記

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序