產(chǎn)品編號	bsm-33187M
英文名稱	Mouse Anti-alpha smooth muscle Actin antibody
中文名稱	肌動蛋白α/α-SMA/α Actin單克隆抗體
別名	alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin;
	Specific References (11) \| bsm-33187M has been referenced in 11 publications. [IF=10.435] Mei, Jiawei. et al. An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22 IHC ; Mouse. PubMed:35568914 [IF=6.107] Lianglong Chen. et al. Multifunctional sponge scaffold loaded with concentrated growth factors for promoting wound healing. ISCIENCE. 2023 Jan;26:105835 IHC ; Rat. PubMed:36624841 [IF=5.696] Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692 IF ; Human. PubMed:34153644 [IF=4.967] Weijie Jiao. et al. Construction and Evaluation of Small-Diameter Bioartificial Arteries Based on a Combined-Mold Technology. POLYMERS-BASEL. 2022 Jan;14(15):3089 IF ; Rat. PubMed:35956602 [IF=4.101] Zengxian Sun. et al. Metformin inhibits pulmonary artery smooth muscle cell proliferation by upregulating p21 via NONRATT015587.2. Int J Mol Med. 2022 Apr;49(4):1-13 FC ; Rat. PubMed:35147202 [IF=4.068] Ming Li. et al. ADAMTS12, a novel prognostic predictor, promotes cell proliferation, migration and invasion in head and neck squamous cell carcinoma. ORAL DIS. 2022 Oct;: IF ; Human. PubMed:36222542 [IF=3.61] Lu X. et al. Anti-renal fibrosis effect of asperulosidic acid via TGF-β1/smad2/smad3 and NF-κB signaling pathways in a rat model of unilateral ureteral obstruction IHC ; human. PubMed:doi:10.1016/j.phymed.2018.09.009 [IF=3.067] Liyan Shi. et al. Cranberry (Vacinium macrocarpon) phytochemicals inhibit hepatic stellate cell activation and liver fibrosis. Food Biosci. 2021 Aug;42:101176 IF ; Rat. PubMed:10.1016/j.fbio.2021.101176 [IF=3.046] Tan HX et al. TGFβ1 is essential for MSCs-CAFs differentiation and promotes HCT116 cells migration and invasion via JAK/STAT3 signaling. Onco Targets Ther. 2019 Jul 5;12:5323-5334. FCM ; Human. PubMed:31308702 [IF=2.751] Ziqian Liu. et al. Eplerenone ameliorates lung fibrosis in unilateral ureteral obstruction rats by inhibiting lymphangiogenesis. EXP THER MED. 2022 Oct;24(4):1-12 IHC ; Rat. PubMed:10.3892/etm.2022.11560 [IF=1.56] Jin, Yingli, et al. "Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method." Molecular Medicine Reports. IHC-P ; Rat. PubMed:28075469
研究領(lǐng)域	細(xì)胞生物發(fā)育生物學(xué) 細(xì)胞骨架
抗體來源	Mouse
克隆類型	Monoclonal
克隆號	3F9
交叉反應(yīng)	Human,Mouse,Rat (predicted: Chicken)
產(chǎn)品應(yīng)用	WB=1:500-5000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	42kDa
細(xì)胞定位	細(xì)胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human Actin alpha
亞型	IgG
純化方法	affinity purified by Protein G
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008] Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Subcellular Location: Cytoplasm, cytoskeleton. Post-translational modifications: Oxidation of Met-46 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced (By similarity). DISEASE: Note=ACTA2 mutations predispose patients to a variety of diffuse and diverse vascular diseases, premature onset coronary artery disease (CAD), premature ischemic strokes and Moyamoya disease. Defects in ACTA2 are the cause of familial aortic aneurysm thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Defects in ACTA2 are the cause of Moyamoya disease type 5 (MYMY5) [MIM:614042]. Moyamoya disease is a progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia. The abnormal vessels resemble a 'puff of smoke' (moyamoya) on cerebral angiogram. Affected individuals can develop transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage. Hemiplegia of sudden onset and epileptic seizures constitute the prevailing presentation in childhood, while subarachnoid bleeding occurs more frequently in adults. Defects in ACTA2 are the cause of multisystemic smooth muscle dysfunction syndrome (MSMDYS) [MIM:613834]. MSMDYS is a syndrome characterized by dysfunction of smooth muscle cells throughout the body, leading to aortic and cerebrovascular disease, fixed dilated pupils, hypotonic bladder, malrotation, and hypoperistalsis of the gut and pulmonary hypertension. Similarity: Belongs to the actin family. SWISS: P62736 Gene ID: 59 Database links: Entrez Gene: 101021287 Baboon Entrez Gene: 515610 Cow Entrez Gene: 59 Human Entrez Gene: 11475 Mouse Entrez Gene: 733615 Pig Entrez Gene: 100009271 Rabbit Entrez Gene: 81633 Rat Omim: 102620 Human SwissProt: P62739 Cow SwissProt: P62736 Human SwissProt: P62737 Mouse SwissProt: P62740 Rabbit SwissProt: P62738 Rat Unigene: 500483 Human Unigene: 213025 Mouse Unigene: 195319 Rat Unigene: 3114 Rat 結(jié)構(gòu)蛋白（Structural Proteins） Actin α/α-Actin 是一種具有收縮能力的微絲蛋白，a-SMA廣泛分布于幾乎所有的肌型細(xì)胞中。Actin-α蛋白主要用于檢測骨骼肌、平滑肌、血管平滑肌、心肌和肌原性腫瘤包括：平滑肌瘤、平滑肌肉瘤、橫紋肌肉瘤以及肌上細(xì)胞和肌上皮瘤。Actin（肌動蛋白）是在所有真核細(xì)胞中都表達(dá)的高度保守的蛋白質(zhì)。它們沿微管組成了細(xì)胞骨架的主要成分。肌動蛋白至少表達(dá)為6種異構(gòu)形式。它在心臟、骨骼橫紋肌組織和某些平滑肌組織中表達(dá)，調(diào)節(jié)其收縮功能。有報導(dǎo)說肌動蛋白在乳房瘤中是高度磷酸化的。肌動蛋白的功能失調(diào)也會導(dǎo)致某種類型的心臟病。平滑肌α肌動蛋白使人更感興趣，因為編碼它的基因是相對局限于在血管平滑肌細(xì)胞中表達(dá)的少數(shù)幾個基因之一。肌動蛋白是標(biāo)記平滑肌和肌上皮細(xì)胞腫瘤的有效工具。
產(chǎn)品圖片	Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug Lane 2: Stomach (Mouse) Lysate at 40 ug Lane 3: Uterus (Mouse) Lysate at 40 ug Lane 4: Kidney (Mouse) Lysate at 40 ug Lane 5: Small intestine (Mouse) Lysate at 40 ug Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 7: Stomach (Rat) Lysate at 40 ug Lane 8: Uterus (Rat) Lysate at 40 ug Lane 9: A549 (Human) Cell Lysate at 30 ug Lane 10: Hela (Human) Cell Lysate at 30 ug Lane 11: A431 (Human) Cell Lysate at 30 ug Lane 12: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-alpha smooth muscle Actin (bsm-33187M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (Human uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha smooth muscle Actin) Monoclonal Antibody, Unconjugated (ascites of bsm-33187M 3F9) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with alpha smooth muscle Actin Monoclonal Antibody, Unconjugated (bsm-33187M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:NIH/3T3. Primary Antibody (green line): Mouse Anti-alpha smooth muscle Actin antibody (bsm-33187M) Dilution: 1ug/Test; Secondary Antibody : Goat anti-mouse IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	AbBy Fluor 350 標(biāo)記
	AbBy Fluor 488 標(biāo)記
	AbBy Fluor 555 標(biāo)記
	AbBy Fluor 594 標(biāo)記
	AbBy Fluor 647 標(biāo)記
	AP 標(biāo)記
	APC 標(biāo)記
	Biotin 標(biāo)記
	Cy3 標(biāo)記
	Cy5 標(biāo)記
	Cy5.5 標(biāo)記
	Cy7 標(biāo)記
	FITC 標(biāo)記
	Gold 標(biāo)記
	HRP 標(biāo)記
	PE 標(biāo)記
	PE-Cy3 標(biāo)記
	PE-CY5 標(biāo)記
	PE-CY5.5 標(biāo)記
	PE-CY7 標(biāo)記
	RBITC 標(biāo)記

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價測定程序