| 產品編號 | C01-01014 |
| 英文名稱 | HEPES buffer, 2×(pH7.2-7.4, sterile) |
| 中文名稱 | Hepes Buffer (2×,pH7.2-7.4,無菌) |
| 別 名 | 2×HEPES buffer(pH7.2-7.4，sterile); 2 × HEPES buffer(pH7.2-7.4，sterile); 2-[4-(羥乙基)-1-哌嗪基]乙磺酸;N-(2-羥乙基)哌嗪-N'-乙烷磺酸;4-羥乙基哌嗪乙磺酸;N-(2-羥乙基)哌嗪-N'-2-乙烷磺酸;4-(2-羥乙基)-1-哌嗪乙磺酸;N-2-羥乙基哌嗪-N'-2-乙磺酸(HEPES);羥乙基哌嗪乙磺酸;2-[4-(2-羥乙基)-1-哌嗪乙烷]磺酸; 2×HEPES(pH7.2- 7.4); HEPES buffer, 2X(pH7.2-7.4, sterile); |
| 保存條件 | Store at 2-8℃ for one year. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. Application HEPES has been used as a component of: Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices. Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity. General description: HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. Hepes是一種氫離子緩沖劑，能較長時間控制恒定的pH范圍，有效緩沖范圍為pH6.8-8.2?？捎糜谂渲频鞍滋崛【彌_液、細胞培養(yǎng)用緩沖液等，對細胞無毒性作用。使用終濃度為10-50mmol/L，一般培養(yǎng)液內含20mmol/LHEPES即可達到緩沖能力，細胞培養(yǎng)常用終濃度為 5-30mM/L。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
