| 產(chǎn)品編號(hào) | bsm-51396M |
| 英文名稱 | Mouse Anti-Topoisomerase I antibody |
| 中文名稱 | 拓普西異構(gòu)酶Ⅰ單克隆抗體 |
| 別 名 | DNA topoisomerase 1; DNA topoisomerase I; NUP98 fusion gene; TOP 1; TOP I; TOP1; TOP1_HUMAN; TOPI; Topoisomerase 1; Topoisomerase1; TopoisomeraseI; Type I DNA topoisomerase. |
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Specific References (1) | bsm-51396M has been referenced in 1 publications.
[IF=3.407] Xu W et al. Tetrandrine?and?cepharanthine?induce?apoptosis?through?caspase?cascade?regulation,?cell?cyclearrest,?MAPK?activation?and?PI3K/Akt/mTOR?signal?modification?in glucocorticoid resistant human leukemia Jurkat T cells. Chem Biol Interact.?2019 Jun 28;310:108726. WB ; Human.
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| 研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號(hào) |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 3D7 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Topoisomerase I |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] Function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. Subcellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. Post-translational modifications: Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. DISEASE: Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Similarity: Belongs to the eukaryotic type I topoisomerase family. SWISS: P11387 Gene ID: 7150 Database links: Entrez Gene: 7150 Human Entrez Gene: 21969 Mouse Omim: 126420 Human SwissProt: P11387 Human SwissProt: Q04750 Mouse Unigene: 472737 Human Unigene: 712686 Human Unigene: 217233 Mouse Unigene: 91572 Rat |
| 產(chǎn)品圖片 |
Sample:
MOLT-4 (Human) Cell Lysate at 30 ug
MCF-7 (Human) Cell Lysate at 30 ug
SiHa (Human) Cell Lysate at 30 ug
K562 (Human) Cell Lysate at 30 ug
Primary: Anti-Topoisomerase I (bsm-51396M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
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