| 產(chǎn)品編號 | C5076 |
| 英文名稱 | Lowry Protein Assay Kit |
| 中文名稱 | Lowry法蛋白濃度測定試劑盒（1000T） |
| 別 名 | Protein Quantitation Assay; Modified Lowry Protein Assay Kit; Protein Quantitation Assay; 穩(wěn)定型Lowry法蛋白濃度測定試劑盒; 改良型Lowry法蛋白濃度測定試劑盒 |
| 保存條件 | 試劑盒2-8℃保存，標準品-20℃保存，開封使用后請密封保存. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
The folin phenol reagent method consists of two steps: the first step is protein interact with copper to form protein-copper complex in an alkaline solution. The second step is the complex reduce the Folin reagent to produce dark blue. The amount of color produced is proportional to protein concentration. The quantitative rang of protein is 5~100μg/ml. Folin reagent chromogenic reaction is caused by tyrosine, tryptophan and cysteine. Therefore, phenols, citric acid and sulfhydryl compounds in the sample have interfere. Besides, different proteins have different color intensity due to different contents of tyrosine and tryptophan. Lowry法包括兩步反應：第一步是在堿性條件下，蛋白質(zhì)與銅作用生成蛋白質(zhì)-銅絡合物；第二步是此絡合物將Folin試劑還原，產(chǎn)生深藍色，顏色深淺與蛋白質(zhì)含量成正比。定量范圍為5～100μg/ml蛋白質(zhì)。Folin試劑顯色反應由酪氨酸、色氨酸和半胱氨酸引起，因此樣品中若含有酚類、檸檬酸和巰基化合物均有干擾作用。此外，不同蛋白質(zhì)因酪氨酸、色氨酸含量不同而使顯色強度稍有不同。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
