| 產(chǎn)品編號 | bsm-51668M |
| 英文名稱 | Mouse Anti-ADK antibody |
| 中文名稱 | 腺苷酸激酶單克隆抗體 |
| 別 名 | Adenosine kinase; AK; EC:2.7.1.20; Adenosine 5'-phosphotransferase; ADK_HUMAN; |
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | N3W5 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-4000,IHC-P=1:25,IHC-F=1:25,IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human ADK between 1-345 amino acids. |
| 亞 型 | IgG1,k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] Function: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Subunit: Monomer. Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney. DISEASE: Hypermethioninemia due to adenosine kinase deficiency (HMAKD) [MIM:614300]: A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the carbohydrate kinase PfkB family. SWISS: P55263 Gene ID: 132 Database links: Entrez Gene: 132 Human SwissProt: P55263 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: HepG2 cell lysates
Lane 2: HL-60 cell lysates
Lane 3: Human liver tissue lysates
Primary: Anti-ADK (bsm-51668M) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (human kidney tissue sections ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADK) Monoclonal Antibody, Unconjugated (bsm-51668M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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