| 產(chǎn)品編號 | bsm-51712M |
| 英文名稱 | Mouse Anti-HLA-F antibody |
| 中文名稱 | 組織相容性抗原HLAF單克隆抗體 |
| 別 名 | CDA12; HLA 5.4; HLA class I histocompatibility antigen, alpha chain F; HLA class I histocompatibility antigen, alpha chain F; HLA class I molecule; HLA F antigen; HLA-CDA12; HLA-F; HLAF; HLAF_HUMAN; Leukocyte antigen F; MHC class I antigen F. |
| 研究領域 | 免疫學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | H9Y7 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human HLA-F. |
| 亞 型 | IgG1,Κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008] Function: Involved in the presentation of foreign antigens to the immune system. Subunit: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Subcellular Location: Membrane.. Similarity: Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain. SWISS: P30511 Gene ID: 3134 Database links: Entrez Gene: 3134 Human SwissProt: P30511 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Raji cell lysates
Primary: Anti-HLA-F (bsm-51712M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 42 kD
Sample:
Lane 1: HepG2 cell lysates
Lane 2: HL-60 cell lysates
Primary: Anti-HLA-F (bsm-51712M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 42 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
