| 產(chǎn)品編號 | bsm-60525R |
| 英文名稱 | PRMT5 Recombinant Rabbit mAb |
| 中文名稱 | 組蛋白精氨酸甲基轉移酶5重組兔單抗 |
| 別 名 | ANM5_HUMAN; Protein arginine N-methyltransferase 5; HRMT1L5; IBP72; JBP1; SKB1; EC:2.1.1.320; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog(SKB1 homolog; SKB1Hs); Protein arginine N-methyltransferase 5, N-terminally processed; protein arginine methyltransferase 5; HSL7; SKB1Hs; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | D1B12 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:2000-1:10000,IHC-P=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 70 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] 精氨酸甲基轉移酶，既能催化omega-N單甲基精氨酸（MMA）和對稱二甲基精氨酸（sDMA）的形成，又能優(yōu)先催化MMA的形成。特異性介導小核核糖核蛋白smd1（SNRPD1）和smd3（SNRPD3）中精氨酸殘基的對稱二甲基化；這種甲基化是snRNP核心粒子組裝和生物生成所必需的。甲基化SUPT5H并可能調(diào)節(jié)其轉錄延伸特性。髓鞘堿性蛋白（MBP）精氨酸殘基的單酸和二甲基化?？赡茉诩毎蜃蛹せ畹霓D導途徑中發(fā)揮作用。負調(diào)控細胞周期素E1啟動子活性和細胞增殖。在生殖細胞發(fā)育過程中甲基化組蛋白H2A和H4'Arg-3'。甲基化組蛋白H3'Arg-8'，可能抑制轉錄。甲基化Piwi蛋白（PIWIL1，PIWIL2和PIWIL4），Piwi蛋白甲基化是與包含Tudor結構域的蛋白質相互作用和隨后定位到減數(shù)分裂的細微差別所必需的。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:10000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: HEK-293, 3: MCF7, 4: HepG2
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 73 kDa
Observed MW: 70 kDa
Tissue: Human breast cancer
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60525R
Tissue: Human liver
Section type: Formalin-fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60525R
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
