| 產(chǎn)品編號(hào) | bsm-60406R |
| 英文名稱(chēng) | TIA1 Recombinant Rabbit mAb |
| 中文名稱(chēng) | |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | D1D5 |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000,Flow-Cyt=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 通過(guò)與位于mRNA 3'非翻譯區(qū)（3'UTRs）的富AU元素（AREs）結(jié)合，參與RNA前體選擇性剪接和mRNA翻譯調(diào)控。對(duì)細(xì)胞毒性淋巴細(xì)胞靶細(xì)胞具有溶核活性?？赡軈⑴c細(xì)胞凋亡。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: Jurkat, 3: K562, 4: MOLT-4, 5: NIH-3T3
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 43 kDa
Observed MW: 42-43 kDa
Cell line: Jurkat
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60406R
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