| 產(chǎn)品編號(hào) | bs-60081R |
| 英文名稱 | Rabbit Anti-Histone H3 (tri methyl K4, serotonyl Q5) antibody |
| 中文名稱 | |
| 產(chǎn)品類型 | 甲基化抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾（PTM）是表觀遺傳學(xué)的關(guān)鍵機(jī)制，可調(diào)節(jié)染色質(zhì)結(jié)構(gòu)，稱為“組蛋白密碼”。血清素也稱為5-羥色胺（5-HT），是一種興奮性神經(jīng)遞質(zhì)，可激活大腦中特定的膜受體來調(diào)節(jié)腦細(xì)胞的活動(dòng)。在TGM2蛋白的催化下，5-羥色胺的氨基可與組蛋白H3第5位谷氨酰胺的側(cè)鏈羧基共價(jià)結(jié)合，形成組蛋白5-羥色胺（H3Q5ser）。H3Q5ser與相鄰的H3K4me3間相互影響。H3K4me3Q5ser可增強(qiáng)TFIID等轉(zhuǎn)錄因子與染色質(zhì)的結(jié)合，從而激活發(fā)育中的嚙齒動(dòng)物大腦或人類神經(jīng)元中下游基因的表達(dá)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: lane 1: PTM-1420+H3K4me3/Q5ser peptides, lane 2: PTM-1420+unmodified peptides, lane 3: PTM-1420, 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: N2a
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
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