| 產(chǎn)品編號(hào) | bsm-60478R |
| 英文名稱 | MPP8 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | H12G4 |
| 交叉反應(yīng) | Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 異染色質(zhì)組分，特異性識(shí)別和結(jié)合組蛋白H3（H3K9me）的甲基化“Lys-9”，并促進(jìn)介導(dǎo)表觀遺傳抑制的蛋白質(zhì)的募集。介導(dǎo)HUSH復(fù)合體向H3K9me3位點(diǎn)的募集：HUSH復(fù)合體被募集到富含H3K9me3的基因組位點(diǎn)，并且需要通過(guò)促進(jìn)SETDB1（一種介導(dǎo)H3K9me3進(jìn)一步沉積的組蛋白甲基轉(zhuǎn)移酶）以及MORC2的募集來(lái)維持轉(zhuǎn)錄沉默。結(jié)合H3K9me并通過(guò)招募DNMT3A到靶CpG位點(diǎn)來(lái)促進(jìn)DNA甲基化；它們可以位于基因的編碼區(qū)內(nèi)。介導(dǎo)CDH1表達(dá)下調(diào)。同時(shí)與MORC2和SETDB1協(xié)同抑制L1逆轉(zhuǎn)錄轉(zhuǎn)座子，沉默依賴于抑制性表觀遺傳修飾，如H3K9me3標(biāo)記。沉默事件通常發(fā)生在轉(zhuǎn)錄活躍基因的內(nèi)含子內(nèi)，導(dǎo)致宿主基因表達(dá)下調(diào)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: MEF, 2: Mouse E12 brain, 3: 4T1, 4: F9
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 110 kDa
Observed MW: 125 kDa
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