| 產(chǎn)品編號 | bsm-60433R |
| 英文名稱 | CLDN1 Recombinant Rabbit mAb |
| 中文名稱 | 緊密連接蛋白1重組兔單抗 |
| 別 名 | CLD1_HUMAN; Claudin-1; Claudin 1; Claudin1; CLD1; SEMP1; Senescence-associated epithelial membrane protein; UNQ481/PRO944; ILVASC; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | B5D10 |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Claudin 1: 180-211 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008] Subcellular Location: Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed, with highest levels in liver and kidney. Database links: Entrez Gene: 9076 Human Entrez Gene: 12737 Mouse SwissProt: O95832 Human SwissProt: O88551 Mouse claudins充當(dāng)緊密連接復(fù)合物的主要成分，該緊密連接復(fù)合物調(diào)節(jié)上皮的通透性。盡管某些claudin家族成員在不可滲透屏障的形成中起著至關(guān)重要的作用，但其他一些介導(dǎo)了對離子和小分子的滲透性。通常，幾個claudin家族成員會共表達(dá)并相互影響，這決定了總體滲透性。 CLDN1是必需的，可防止小分子通過表皮中的緊密連接發(fā)生旁細(xì)胞擴(kuò)散，而CLDN1是皮膚的正常屏障功能所必需的。正常的水體內(nèi)平衡和防止水通過皮膚過多流失可能是必需的，這可能是通過間接影響其他蛋白質(zhì)的表達(dá)水平來實現(xiàn)的，因為CLDN1本身似乎對角質(zhì)形成細(xì)胞緊密連接中的水屏障形成不可或缺。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CLDN1 monoclonal antibody, unconjugated (bsm-60433R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with CLDN1 monoclonal antibody, unconjugated (bsm-60433R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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