| 產(chǎn)品編號 | bsm-0474M |
| 英文名稱 | human IgA Mouse mAb |
| 中文名稱 | 人血清型免疫球蛋白A抗體 |
| 別 名 | Native Human IgA protein; Human IgA protein; IgA1; Igh2; IGHA 1; IGHA 2; IGHA1; IGHA2; Immunoglobulin alpha 1; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin heavy chain 2(serum IgA); Immunoglobulin heavy constant alpha 1; Immunoglobulin heavy constant alpha 2; Human Immunoglobulin A; human Serum IgA; |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 7H8 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | IHC-P=1:500-1500,IHC-F=1:500-1500,IF=1:500-1500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 免 疫 原 | purified human Serum IgA |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. Subcellular Location: Secreted. SWISS: P01876 Gene ID: 3493 IgA有IgA1和IgA2兩個亞類。IgA1主要存在于血清中，約占血清中IgA的85%，α1鏈分子量為56kD；IgA2主要存在于外分泌液中，少部分以血清型IgA存在，約占血清中IgA的15%，α2鏈缺乏鉸鏈區(qū)，分子量為52kD。血清中的IgA除單體形式外還有由J鏈共價相連的二聚體或三聚體等形式。分泌型IgA是由J連接的雙體和分泌成分所組成，主要存在于初乳、唾液、淚液、胃腸液、支氣管分泌等外分泌液中，是粘膜局部免疫的最重要因素，分泌型IgA通過與相應(yīng)的病原微生物（如脊髓灰質(zhì)炎病毒）結(jié)合，阻抑其吸附到易感細胞上，分泌型IgA還可中和毒素如霍亂弧菌毒素和大腸桿毒素等。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human IgA ) Monoclonal Antibody, Unconjugated (bsm-0474M) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (human IgA) Monoclonal Antibody, Unconjugated (bsm-0474M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
