| 產(chǎn)品編號 | bsm-41335M |
| 英文名稱 | LAMP1 Mouse mAb |
| 中文名稱 | 溶酶體相關膜蛋白1（CD107）單克隆抗體 |
| 別 名 | CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 4; |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 免 疫 原 | Recombinant human LAMP1 protein: 29-382/417 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS(pH7.4) |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. Function: Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Post-translational modifications: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. Similarity: Belongs to the LAMP family. Database links: Entrez Gene: 3916 Human Entrez Gene: 16783 Mouse Omim: 153330 Human SwissProt: P11279 Human SwissProt: P11438 Mouse Unigene: 494419 Human Unigene: 16716 Mouse Unigene: 475822 Mouse Unigene: 40177 Rat CD107a可很好的反映NK細胞活性，其表達水平的上調與NK分泌細胞因子的增多及NK對靶細胞的殺傷作用的增強高度相關，采用流式檢測，CD107a還可鑒定出一群無細胞因子分泌的活性NK細胞。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with LAMP1 monoclonal antibody, unconjugated (bsm-41335M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated(bsm-41335M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated(bsm-41335M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated(bsm-41335M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated(bsm-41335M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated(bsm-41335M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated(bsm-41335M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (LAMP1) monoclonal Antibody, unconjugated (bsm-41335M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated (bsm-41335M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LAMP1 Monoclonal Antibody, Unconjugated (bsm-41335M) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-41335M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HT-1080 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-LAMP1 antibody (bsm-41335M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
